Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters pigment production in the plant
Reexamination Certificate
2006-12-14
2011-12-06
Kallis, Russell (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters pigment production in the plant
C800S306000, C800S312000, C800S314000, C800S317400, C800S320000, C800S320100, C800S320200, C800S320300, C536S023100, C435S419000
Reexamination Certificate
active
08071841
ABSTRACT:
The cauliflower (Brassica oleraceaL. var.botrytis) Or gene is a semi-dominant, single-locus mutation. It induces the accumulation of high levels of beta-carotene in various tissues that are normally devoid of carotenoids, turning them orange. Using a map-based cloning strategy, we identified a single gene representing Or and successfully verified its identity by functional complementation in the wild type cauliflower. The Or gene encodes a plastid membrane protein containing the DnaJ zinc figure domain. A likely gain-of-function mutation from a 4.3-kb retrotransposon insertion in the Or allele confers the orange phenotype in the mutant. Southern blot analysis revealed that Or is a single-copy sequence in the cauliflower genome. High level of expression of the Or gene and the protein was found in very young leaves, curds, and flowers at comparable abundance between wild type and the Or mutant. Or likely functions in regulating the differentiation of some non-photosynthetic plastids into chromoplasts, which provide the deposition “sink” for carotenoid accumulation. Successful demonstration of Or in conferring carotenoid accumulation in potato tubers indicates its potential use to improve the nutritional value in staple crops.
REFERENCES:
Lu, S. et al. The Plant Cell, 2006; vol. 18, pp. 3594-3605.
Li Li
Van Eck Joyce
Fadp John D.
Kallis Russell
Rabin Evelyn M.
The United States of America as represented by the Secretary of
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