Imperforate bowl: centrifugal separators – Including plurality of miniature bowls distrubuted about...
Patent
1993-06-18
1995-10-24
Jenkins, Robert W.
Imperforate bowl: centrifugal separators
Including plurality of miniature bowls distrubuted about...
494 37, B04B 502
Patent
active
RE0350710
ABSTRACT:
A centrifuge rotor and method for gradient density separation which supports a generally cylindrical volume of sample solution with its axis inclined to the spin axis at an angle which is optimized for maximum separation efficiency while reducing contamination by undesirable centrifugates upon reorientation of the desirable centrifugates. The sample solution is contained in a centrifuge tube which is closed at its top end by a top portion which is supported by a support cap. The undesirable centrifugates are pelleted to the end corners of the inclined centrifuge tube. The rotor and method are particularly adapted, for example, for separation of nucleic acid into plasmid DNA and chromosomal DNA by density gradient centrifugation. The optimum angle is determined based on the relationship .theta.=Tan.sup.-1 (D/15L).sup.0.5 where D and L are respectively the diameter and length of the cylindrical volume of the sample solution and .theta. is the angle of inclination.
REFERENCES:
patent: 3998383 (1976-12-01), Romanauskas et al.
patent: 4015775 (1977-04-01), Rohde
patent: 4290550 (1981-09-01), Chulay et al.
patent: 4301963 (1981-11-01), Nielsen
patent: 4304356 (1981-12-01), Chulay et al.
patent: 4509940 (1985-04-01), Romanauskas
patent: 4690670 (1987-09-01), Nielsen
patent: 4692137 (1987-09-01), Anthony
patent: 4824429 (1989-04-01), Keunen
Flamm, W. G. et al.; "Density-Gradient Centrifugation of DNA In a Fixed-Angle Rotor, A Higher Order of Resolution" Biochimica et Biophysica ACTA, 129 (1966) pp. 310-317.
Fisher, W. D. et al.; "Density Gradient Centrifugation in Angle-Head Rotors"; Analytical Biochemistry 9 (1964) pp. 477-482.
Masket, A. Victor; "A Quantity Type Rotor for the Ultracentrifuge"; Review of Scientific Instruments pp. 277-279 (undated).
Lewis Mark L.
Little Stephen E.
Sharples Thomas D.
Beckman Instruments Inc.
Hampson Gary T.
Jenkins Robert W.
May William H.
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