Imperforate bowl: centrifugal separators – Including plurality of miniature bowls distrubuted about...
Patent
1989-10-06
1991-06-18
Jenkins, Robert W.
Imperforate bowl: centrifugal separators
Including plurality of miniature bowls distrubuted about...
494 37, B04B 502
Patent
active
050246461
ABSTRACT:
A centrifuge rotor and method for gradient density separation which supports a generally cylindrical volume of sample solution with its axis inclined to the spin axis at an angle which is optimized for maximum separation efficiency while reducing contamination by undesirable centrifugates upon reorientation of the desirable centrifugates. The optimum angle is determined based on the relationship .theta.=Tan.sup.-1 (D/15L).sup.0.5 where D and L are respectively the diameter and length of the cylindrical volume of the sample solution and .theta. is the angle of inclination.
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Flamm, W. G. et al.; "Density-Gradient Centrifugation of DNA In a Fixed-Angle Rotor", A Higher Order of Resolution Biochimica et Biophysica Acta, 129 (1966) pp. 310-317.
Fisher, W. D. et al.; "Density Gradient Centrifugation in Angle-Head Rotors"; Analytical Biochemistry 9 (1964) pp. 477-482.
Masket, A. Victor; "A Quantity Type Rotor for the Ultracentrifuge"; Review of Scientific Instruments pp. 277-279.
Lewis Mark L.
Little Stephen E.
Sharples Thomas D.
Beckman Instruments Inc.
Harder Paul R.
Jenkins Robert W.
May William H.
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