Optimized recognition site of the alphavirus non-structural...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C536S023100

Reexamination Certificate

active

11157360

ABSTRACT:
The present invention discloses highly efficient novel recognition sites for Pro39 protease. The invention further provides a wide range of conditions for protein purification and modification with the novel recognition sites. The invention even further provides expression vectors for expression of fusion proteins in cells and method to purify fusion proteins.

REFERENCES:
patent: WO 92/10578 (1992-06-01), None
Kohno et al., Semliki Forest virus-based DNA expression vector: transient protein production followed by cell death, Gene Therapy (1998), 5, p. 415-418.□□.
Liljestrom et al., A new generation of animal cell expression vectors based on the Semliki Forest virus replicon, Bio/Technology Dec. 9, (1991), 1356-1361.
Vasiljeva L. et al. 2000 Identification of Novel function of the alphavirus capping . . . J. of Biol. Chem. 275(23): 1728-1787.
Vasiljeva L et al. 2001. Site-specific protease activity of hte carboxyl . . . J. of Biol Chem. 276(33) 30786-30793.
Merits A. et al. 2001. Proteolytic processing of Semliki Forest Virus . . . J. Gen Vir. 82: 765-773.

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