Optimized primer library for gene sequencing and method of using

Details Optimized primer library for gene sequencing and method of using Optimized primer library for gene sequencing and method of using

1997-05-21

2000-07-04

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

060836954

ABSTRACT:
A novel method of primer walking using octamer oligonucleotides to prime DNA sequencing reactions is described. Octamer sequencing is compatible with isotopic and fluorescent sequencing chemistry, reaction conditions are optimized such that the samples can be processed in parallel and the procedures are automated. This strategy is faster than the traditional primer walking sequencing strategy as the existence of a primer library allows immediate access to a primer for the next sequencing reaction, eliminating delays associated with designing and synthesizing gene specific primers. The octamer library is comprised of optimized sequencing primers, such that octamer sequencing yields results equivalent to or better than traditional primer walking.

REFERENCES:
patent: 5407799 (1995-04-01), Studier
Burbelo et al. Biotechnique (1994) 16(4): 645-650.

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