Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof
Reexamination Certificate
2007-10-02
2007-10-02
Salimi, Ali R. (Department: 1648)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Virus or component thereof
C424S199100, C435S091100, C435S320100
Reexamination Certificate
active
10551057
ABSTRACT:
Synthetic DNA molecules encoding the HPV31 L1 protein are provided. Specifically, the present invention provides polynucleotides encoding HPV31 L1 protein, wherein said polynucleotides are free from internal transcription termination signals that are recognized by yeast. Also provided are synthetic polynucleotides encoding HPV31 L1 wherein the polynucleotides have been codon-optimized for high level expression in a yeast cell. The synthetic molecules may be used to produce HPV31 virus-like particles (VLPs), and to produce vaccines and pharmaceutical compositions comprising the HPV31 VLPs. The vaccines of the present invention provide effective immunoprophylaxis against papillomavirus infection through neutralizing antibody and cell-mediated immunityHPV31 L1 total rebuild nucleotide and aminoacid sequence: M S L W R P S 1 ATGTCTTTGT GGAGACCATC E A T V Y L P P V PTGAAGCTACC GTCTACTTGC CACCAGTCCC V S K V V S T 51 AGTCTCTAAG GTCGTCTCTA D E Y V T R T N I YCCGACGAATA CGTCACCAGA ACCAACATCT Y H A G S A 101 ACTACCACGC TGGTTCTGCTR L L T V G H P Y YAGATTGTTGA CCGTCGGTCA CCCATACTAC S I P K S D N 151 TCTATCCCAA AGTCTGACAA P K K I V V P K V SCCCAAAGAAG ATCGTCGTCC CAAAGGTCTC G L Q Y R V F 201 TGGTTTGCAA TACAGAGTCT R V R L P D P N K FTCAGAGTCAG ATTGCCAGAC CCAAACAAGT G F P D T S 251 TCGGTTTCCC AGACACCTCTF Y N P E T Q R L VTTCTACAACC CAGAAACCCA AAGATTGGTC W A C V G L E 301 TGGGCTTGTG TCGGTTTGGA V G R G Q P L G V GAGTCGGTAGA GGTCAACCAT TGGGTGTCGG I S G H P L L 351 TATCTCTGGT CACCCATTGT N K F D D T E N S NTGAACAAGTT CGACGACACC GAAAACTCTA R Y A G G P 401 ACAGATACGC TGGTGGTCCAG T D N R E C I S MGGTACCGACA ACAGAGAATG TATCTCTATG D Y K Q T Q L 451 GACTACAAGC AAACCCAATT C L L G C K P P I GGTGTTTGTTG GGTTGTAAGC CACCAATCGG E H W G K G S 501 TGAACACTGG GGTAAGGGTT P C S N N A I T P GCTCCATGTTC TAACAACGCT ATCACCCCAG D C P P L E 551 GTGACTGTCC ACCATTGGAAL K N S V I Q D G DTTGAAGAACT CTGTCATCCA AGACGGTGAC N V D T G F G 601 ATGGTCGACA CCGGTTTCGG A N D F T A L Q D TTGCTATGGAC TTCACCGCTT TGCAAGACAC K S W V P L D 651 CAAGTCTAAC GTCCCATTGG I C N S I C K Y P DACATCTGTAA CTCTATCTGT AAGTACCCAG Y L K M V A 701 ACTACTTGAA GATGGTCGCTE P Y G D T L F F YGAACCATACG GCGACACCTT GTTCTTCTAC L R R E Q M F 751 TTGCGTAGAG AACAGATGTT V R H F F N R S G TCGTAAGGCAC TTCTTCAACA GATCCGGCAC V G E S V P T 801 CGTAGGTGAA TCTGTCCCAA D L Y I K G S G S TCCGACCTGTA CATCAAGGGC TCCGGTTCCA A T L A N S 851 CCGCTACCCT GGCTAACTCCT Y F P T P S G S NACCTACTTCC CAACTCCATC TGGCTCCATG V T S D A Q I 9
Jansen Kathrin U.
Markus Henry Z.
Neeper Michael P.
Schultz Loren D.
Finnegan Alysia A.
Heber Sheldon O.
Merck & Co. , Inc.
Salimi Ali R.
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