Optimized core promoters and uses thereof

Details Optimized core promoters and uses thereof Optimized core promoters and uses thereof

2011-06-28

2011-06-28

Li, Q. Janice (Department: 1633)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S069100, C435S320100, C424S093210

Reexamination Certificate

active

07968698

ABSTRACT:
The present invention provides core promoter motif ten elements (MTE) and core promoter constructs comprising the MTEs and an initiator element (Inr) in combination with one or both of a TATA box and a downstream promoter element (DPE) which increases gene expression over the strongest known core promoters. Particularly, an optimized or super core promoter is provided which comprises Inr, MTE, TATA box and DPE elements. The present invention also provides expression vectors and host cells comprising the core promoter constructs. Additionally, methods of increasing production of a protein using the core promoter constructs are provided.

REFERENCES:
patent: 5518913 (1996-05-01), Massie et al.
patent: 6124094 (2000-09-01), Lajoie et al.
Kutach et al. Mole Cell Biol 2000;20:4754.
Lim, C. Y. et al.The MTE, a new Core Promoter Element for Transcription by RNA Polymerase II: Genes & Development, 2004, vol. 18, pp. 1606-1617.

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