Optical trap system and method

Details Optical trap system and method Optical trap system and method

1994-03-09

1996-04-30

Anderson, Bruce C.

Radiant energy

Electrically neutral molecular or atomic beam devices and...

H05H 304

Patent

active

055127452

ABSTRACT:
By providing a focal region of light onto a particle, a laser-based light source can provide enough radiation pressure to position the particle at any desired location in space. In one application, the particle can be a micrometer-sized bead, called a handle, attached to a sample. When the sample under examination, such as an actin filament, interacts with other molecules, such as myosin, the forces generated may displace the sample, and thus the handle, out of its original position. To correct for the off-target position (or in other words, to increase the stiffness of the handle), a feedback loop that utilizes a quadrant photodiode detector and a focal region location means such as an acousto-optic modulator or galvanometer mirror is incorporated in the optical trap system. Use of two other light sources for brightfield illumination and epifluorescence allows the simultaneous viewing of the sample in real time. In other embodiments, the optical trap system can trap and manipulate particles.

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Laser Manipulation of Atoms and Particles, Steven Chu, Science, 23 Aug., 1991, vol. 253, pp. 861-866.
"Observation of a Single-Beam Gradient Force Optical Trap for Dielectric Particles", Ashkin et al, Optics Letters, vol. 11, May 1986.
International Conference--Muscle as a Machine: Energy Transduction in the Contractile System, Apr. 13-15, 1992, Lawton Chiles International House.
In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction Using Purified Proteins, Warrick et al., pp. 1-21, Methods In Cell Biology vol. 39, 1993.

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