Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-03-01
2011-03-01
Woolwine, Samuel C (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S003000, C435S069100, C435S091100, C435S091510, C536S023100
Reexamination Certificate
active
07897341
ABSTRACT:
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
REFERENCES:
patent: 4675285 (1987-06-01), Clark et al.
patent: 1522582 (2005-04-01), None
patent: WO-93/03151 (1993-02-01), None
patent: WO-98/41869 (1998-09-01), None
patent: WO-99/02671 (1999-01-01), None
Naoto Nemoto, Etsuko Miyamoto-Sato, Yzuru Husimi, Hiroshi Yanagawa “In vitro virus: Bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro”,Federation of European Biochemical Societies(Aug. 1, 1997) pp. 405-408.
Shane Atwell and James A. Wells “Selection for improved subtiligases by phage display”,Proc. Natl. Acad. Sci. USA(Feb. 26, 1999) vol. 96 pp. 9497-9502.
Jean-Luc Jestin, Peter Dristensen, and Greg Winter, “A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling”Angew.Chem.lnt. (1999) vol. 38, No. 8 pp. 1124-1127.
Patrice Soumillion, Laurent Jespers, Michele Bouchet, Jacqueline Marchand-Brynaert, Greg Winter and Jacques Fastrez “Selection of B-Lactamase on Felamentous Bacteriophage by Catalytic Activity”Accademic Press Limited(1994) vol. 237, pp. 415-422.
Mikael Wildersten and Bengt Mannervik “Glutathione Transferases with Novel active Sites Isolated by Phage Display from a Library of Random Mutants”Academic Press Limited, (1995) vol. 250, pp. 115-122.
Salvator Demartis, Adrian Huber, Francesca Viti, Luisa Lozzi, Leonardo Giovannoni, Paolo Neri, Greg Winter and Dario Neri “A Strategy for the Isolation of Catalytic Actibities from Repertoires of Enzymes Displayed on Phage”Academic Press Limited(1999) Article No. jmbi.1998.2476, pp. 617-633.
Steven Bass, Ronald Greene, and James A. Wells “Hormone Phage: An Enrichment Method for Variant Proteins With Altered Bindig Properties”,Wiley-Liss, (1990), pp. 309-314.
“Filamentous Fusion Phage: Novel Expression vectors That Display cloned Antigens on the Virion Surface”Science, vol. 28 (Jun. 14, 1995), pp. 1315-1317.
Kim D. Janda, Lee-Chiang Lo, Chih-Hung L. Lo, Mui-Mui Sim, Ruo Wang, Chi-Huey Wong, and Richard A. Lerner “Chemical Selection for Catalysis in Combinatorial Antibody Libraries” www.sciencemag.org, vol. 275 (Feb. 14, 1997) pp. 945-948.
John McCafferty, Andrew D. Griffiths, Greg Winter, David J. Chiswell “Phage antibodies: gilamentous phage displaying antibody variable domains”Letters to Nature, vol. 348 (Dec. 6, 1980) pp. 552-554.
Richard W. Roberts and Jack W. Szostak “RNA-peptide fusions for the in vitro selection of peptides and proteins”Proc.Natl.Acad.Sci. USA, vol. 94 (Nov. 1997) pp. 12297-12302.
Millard G. Cull, Jeff F. Miller and Peter J. Schatz “Screening for receptor ligands using large libraries of peptides linked to the C terminus of the lac repressor”Proc.Natl.Acad.Sci. USA, vol. 89, (1992) pp. 1865-1869.
Henrik Pedersen, Swen Holder, Daniel P. Sutherlin, Urs Schwitter, David S. King, and Peter G. Schultz “A method for directed evolution and functional cloning of enzymes”Proc. Natl. Acad. Sci. USA, vol. 95, (Sep. 1998), pp. 10523-10528.
Tawfik, et al., “Man-Made Cell-Like Compartments for Molecular Evolution”, Nature Biotechnology (1998), V. 7, No. 16, pp. 652-656.
Anarbaev, et al., “Klenow Fragment and DNA Polymerase α-Primase Fromserva Calf Thymus in Water-in-Oil Microemulsions”, Biochimica et Biophysica Acta (1998), V. 1384, No. 2, pp. 315-324.
Oberholzer, et al., “Polymerase Chain Reaction in Liposomes”, Chemistry and Biology (1995), V. 2, No. 10, pp. 677-682.
Oberholzer, et al., “Enzymatic RNA Replication in Self-Reproducing Vesicles: An Approach to a Minimal Cell”, Biochemical and Biophysical Research Communications (1995), V. 207, No. 1, pp. 250-257.
Walde, et al., “Oparin's Reactions Revisited: Enzymatic Synthesis of Poly (Adenylic Acid) in Micelles and Self-Reproducing Vesicles”, Journal of the American Chemical Society (1994), V. 116, No. 17, pp. 7541-7547.
Griffiths Andrew
Sepp Armin
Tawfik Dan
Chiang Elbert
Edwards Angell Palmer & & Dodge LLP
Medical Research Council
Williams Kathleen
Woolwine Samuel C
LandOfFree
Optical sorting method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Optical sorting method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Optical sorting method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2625922