Optical method for measuring an analyte using area-modulated lum

Details Optical method for measuring an analyte using area-modulated lum Optical method for measuring an analyte using area-modulated lum

1991-06-05

1993-03-02

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

2504591, 2504612, 356318, 356417, 422 8208, 435 71, 435968, 436172, 436537, 436546, 436800, 436805, G01N 2164, G01N 33542

Patent

active

051908573

ABSTRACT:
A luminescence measuring system is provided for detecting luminescence at extremely low concentrations of luminescing moieties. The method employs alternating radiation at a plurality of loci of an inhomogenous solution, where the radiant power is maintained constant, and the irradiated volumes of pairs of loci are systematically varied. With the probability being very low that the same luminescence signal will be obtained in the two or more measurements, by comparing the measurements, one can detect a low luminescence signal in the presence of relatively high noise levels. Various techniques are described for modulating the irradiance and detecting changes in signal.

REFERENCES:
patent: 4631581 (1986-12-01), Carlsson
Nguyen et al., J. Op. Soc. Am., vol. 4, No. 2, pp. 138-143, Feb. 1987.
Bustamante et al., Proc. Natl. Acad. Sci., vol. 85, pp. 8482-8486, Nov. 1988.

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