Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1998-02-26
2000-12-05
Warden, Jill
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 8205, 422 8209, 436163, 436164, 549223, G01N 2141, G01N 2101, G01N 3116, G01N 2100, C07D31182
Patent
active
061562742
ABSTRACT:
Lipophilic fluorescein-based molecules have been synthesized and incorporated into thin plasticized polymeric membrane films as chromoionophores for the optical sensing and detection of polycationic protamine. The membrane response is based on the extraction of protamine into the film due to the interaction between the anionic fluorescein and the protamine polycation which results in a cation exchange between the protamine and proton, and thus, induces an absorbance spectra change of the polymeric film. The response speed is controlled by protamine diffusion through the stagnant diffusion layer adjacent to the film surface as well as within the bulk of the polymer film. When limited exposure time and non-stirring detection modes are used in a buffer solution, absorbance of a film changes as a function of the protamine concentration in the range of about 2 to 60 .mu.g/ml (0.44 to 13.3 .mu.M). The sensing film shows good selectivity over most common small cations, it can be used in the determination of protamine in diluted serum or whole blood. No response is observed when a protamine complexing reagent such as heparin is present, thus the instant invention is useful as an indicator for the protamine-heparin titration.
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Handy Dwayne K
St. John's University
Warden Jill
LandOfFree
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