Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1995-08-30
1998-09-22
Chin, Christopher L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
356317, 356318, 385 12, 385129, 385130, 422 8205, 422 8208, 422 8211, 4352871, 4352872, 4352887, 435808, 436535, 436164, 436165, 436172, 436805, 436807, G01N 33552
Patent
active
058113126
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to an optical measurement apparatus and method therefor. More particularly, the invention relates to an optical measurement apparatus and method therefor which include a reaction vessel for housing a reagent liquid which includes fluorescent substance, and a test liquid for measurement so as to perform predetermined reaction. A casing constituted the reaction vessel, a part of which casing is formed of an optical waveguide. The apparatus and method measure optical characteristics in the vicinity of a surface of the optical waveguide, (the surface being a surface existing in the reaction vessel) by receiving a fluorescent light component. The fluorescent light component is output of a predetermined relative angle with respect to the optical waveguide due to the fluorescent substance, the fluorescent light being generated by radiating an exciting light into the optical waveguide at a predetermined relative angle with respect to the optical waveguide. Especially preferable, the present invention relates to an optical measurement apparatus and method therefor which excite a fluorescent substance constrained in the vicinity of a surface of an optical waveguide by an evanescent wave component. The evanecent wave component is generated by introducing an exciting light in the optical waveguide so as to propagate in a totally reflective manner. The invention measures the optical characteristics in the vicinity of the surface of the optical waveguide based upon a fluorescent light component which propagates within the optical waveguide in a totally reflective manner, including fluorescent light radiated from the fluorescent substance.
Background Art
In the past, an apparatus for measuring immunity using fluorescent light has been proposed which uses an optical measurement apparatus. The optical measurement apparatus excites a fluorescent substance constrained in the vicinity of a surface of an optical waveguide by an evanescent wave component. The evanescent wave component is generated by introducing an exciting light in the optical waveguide so as to propagate in a totally reflective manner. The optical measurement apparatus then measures optical characteristics in the vicinity of the surface of the optical waveguide based upon a fluorescent light component which propagates within the optical waveguide in a totally reflective manner, from fluorescent light radiated from the fluorescent substance.
Specifically, for example, a reaction vessel is provided which has a face united with a surface of a slab-type optical waveguide. Antibodies (or antigens) are previously fixed on the surface, and a test liquid for measurement and antibodies labeled with fluorescent dye (hereinafter, referred to as labeled antibodies) are poured within the reaction vessel in this order. Then, the labeled antibodies are constrained in the vicinity of the surface by an antigen-antibody reaction. The quantity of the constrained labeled antibodies corresponding to a concentration of antigens in the test liquid for measurement.
The fluorescent dye of the constrained labeled antibodies are excited by an evanescent wave component of the exciting light. The concentration of antigens in the test liquid for measurement is detected based upon an intensity of fluorescent light which is radiated from the fluorescent dye, propagated within the slab-type optical waveguide, and output from the slab-type optical waveguide.
Further, an apparatus for measuring immunity using fluorescent light is conventionally proposed which forms a reaction vessel on one side of an optical waveguide. An immunity reaction is performed between ligands which are made to be a solid phase on the surface of the optical waveguide, a test liquid for measurement which is pored within the reaction vessel, and ligands which are poured within the reaction vessel and which are labeled with a fluorescent dye. The apparatus excites the fluorescent dye by introducing a plane wave into the reaction vessel through the optical waveguide, and me
REFERENCES:
patent: 4775667 (1988-10-01), Saitoh et al.
patent: 4815843 (1989-03-01), Tiefenthaler et al.
patent: 5039490 (1991-08-01), Marsoner et al.
Hasegawa Masanori
Masuda Kenji
Sakamoto Tomomi
Shigemori Kazuhisa
Ueda Tomoaki
Chin Christopher L.
Daikin Industries Ltd.
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