Optical filtering and spectroscopic imaging

Details Optical filtering and spectroscopic imaging Optical filtering and spectroscopic imaging

1995-07-06

1998-08-11

Tokar, Michael J.

Radiant energy

Luminophor irradiation

2504591, G01N 2164

Patent

active

057930490

ABSTRACT:
An imaging scheme for illuminating an object having a plurality of fluors comprising: a light source for simultaneously illuminating the object with light having a plurality of separate excitation bands respectively falling within the fluorescent absorption bands of the fluors; and a detector positioned to receive fluorescent light from the fluors having wavelengths corresponding to the respective fluorescence emission bands of the fluors and adapted to produce a signal representative of the intensity of light received from the object. Also disclosed is an optical filtering scheme for receiving light having a first wavelength distribution and for providing light having a second selected wavelength distribution different from the first wavelength distribution. The above-mentioned imaging and filtering schemes enable simultaneous excitation and detection of a plurality of fluors, while preventing corruption by scattered or reflected excitation light. This enables efficient detection of about 90-95% of the fluorescence emissions from the fluors in the sample.

REFERENCES:
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patent: 4031398 (1977-06-01), Callis et al.
patent: 5051162 (1991-09-01), Kambara et al.
patent: 5192980 (1993-03-01), Dixon et al.
patent: 5294799 (1994-03-01), Aslund et al.
patent: 5418371 (1995-05-01), Aslund et al.
patent: 5424841 (1995-06-01), Van Gelder et al.

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