Optical detection for electronic microarrays

Details Optical detection for electronic microarrays Optical detection for electronic microarrays

2008-03-31

2011-12-27

Kapushoc, Stephen (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S006120, C435S287200, C536S023100

Reexamination Certificate

active

08084202

ABSTRACT:
Embodiments of the invention provide methods for detecting molecular recognition events electronically and optically. Methods according to embodiments of the invention provide a nucleic acid molecule that hybridizes to a first probe nucleic acid molecule attached to an electronic detector wherein the second nucleic acid molecule comprises two regions. The two regions of the second nucleic acid molecule consist of a region that is complementary to the probe nucleic acid and a distal region that is not complementary to the first probe nucleic acid molecule. The hybridization reaction is detected electronically. A third nucleic acid molecule having an attached optically detectable label is hybridized to the distal region of the second nucleic acid molecule and the label is detected optically. Methods according to embodiments of the invention are useful, for example, to validate and quantify electronic detection methods.

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Reynolds et al, Homogeneous nanoparticle based quantitative colrimatric detection of oligonucleotides, 2000, J. Am. Chem. Soc., 122, 3795-3796.
Reynolds et al -Supplemental section 2000, J. Am. Chem. Soc., 122, pp. 1-6.

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