Optical characterization of nucleic acids and oligonucleotides

Details Optical characterization of nucleic acids and oligonucleotides Optical characterization of nucleic acids and oligonucleotides

1997-12-15

2000-07-11

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

060871016

ABSTRACT:
A method for the enzymatic sequencing of DNA is disclosed which uses dideoxythio-nucleotides or dideoxyamino-nucleotides as terminators that are labeled with one or two fluorescent dyes coupled to the terminators either before or after polymerization. The dye labeled DNA fragments are separated in a single lane of a separation system and then the terminal bases of each of the fragments are identified by measuring the fluorescent lifetime of the dye attached to the terminators.

REFERENCES:
patent: 5332666 (1994-07-01), Prober et al.

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