Opposable-element chromatographic assay device for detection of

Details Opposable-element chromatographic assay device for detection of Opposable-element chromatographic assay device for detection of

1997-11-10

2000-07-11

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

422 55, 422 56, 422 58, 422 61, 435 71, 435 75, 435 792, 435 794, 4352871, 4352872, 4352877, 4352879, 435805, 435810, 435962, 435970, 436510, 436512, 436513, 436518, 436524, 436525, 436528, 436531, 436169, 436174, 436805, 436810, G01N 33558

Patent

active

060871849

ABSTRACT:
An assay device for detection or determination of an analyte in a sample uses opposable components and is suitable for assay of human chorionic gonadotropin and other protein or glycoprotein hormones. One embodiment of the device comprises: (1) a first opposable component including: (a) a first chromatographic medium having first and second ends and an immobilized first specific binding partner for the analyte in a detection zone; (b) a conjugate pad in operable contact with the first end of the first chromatographic medium, the conjugate pad containing a labeled second specific binding partner for the analyte in resolubilizable form; and (c) a second chromatographic medium having first and second ends and having immobilized thereon in a reference zone a third specific binding partner that specifically binds the labeled second specific binding partner for the analyte and does not bind the analyte, the first end of the second chromatographic medium being in operable contact with the conjugate pad; and (2) a second opposable component including a sample application zone. The assay device can include a timing control to indicate when flow through the chromatographic medium has occurred and the assay can be read, and can also contain a validation zone ensuring that interference from human anti-mouse antibody is not present. Other embodiments of devices are included, as well as methods of use.

REFERENCES:
patent: 5081013 (1992-01-01), Rovelli et al.
patent: 5308775 (1994-05-01), Donovan et al.

Affiliated with

Also associated with

Rating

Opposable-element chromatographic assay device for detection of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Opposable-element chromatographic assay device for detection of , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Opposable-element chromatographic assay device for detection of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-541392