Opine enhancement of vir gene induction

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352522, 4352536, 4353171, 4352404, 800205, 800DIG63, 935 35, 935 36, 935 38, 935 39, 935 43, 935 55, 935 56, C12N 1563, C12N 1584, A01H 106, A01H 400

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049544420

ABSTRACT:
Transcription of the vir genes of the Ti-plasmid in Agrobacterium species, typically induced by phenolic compounds secreted by wounded plant cells, is enhanced when induction is carried out in the presence of effective amounts of an opine from the Octopine Family of Nopaline Family. Production of T-strands in Agrobacterium species can be potentiated by induction preferably in the presence of octopine, nopaline, succinamopine, or leucinopine. Agrobacterium species induced in the presence of an opine compound of the Nopaline or Octopine Family effect highly efficient transformation of even recalcitrant plant species.

REFERENCES:
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Karen Whiteman Runs Him, Rudy J. Scheffer, and Gary A. Strobel, "Factors Influencing Root Formation in Dicots by Agrobacterium rhizogenes", CAN, J. Bot., vol. 66, 1988.
Shahla N. Sheikholeslam and Donald P. Weeks, "Acetosyringone Promotes High Efficiency Transformation of Arabidopsis thaliana Explants by Agrobacterium tumefaciens", Plant Molecular Biology 8:291-298, (1987).
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