Open-tubular supercritical fluid chromatography

Measuring and testing – Liquid analysis or analysis of the suspension of solids in a... – Content or effect of a constituent of a liquid mixture

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G01N 3108

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044793804

ABSTRACT:
Apparatus for and method of open-tube supercritical fluid chromatography. The apparatus comprises an elongated passageway having inlet and outlet ends such as a capillary column, coated or not with a coating having affinity for solute molecules to be analyzed, means such as a high-pressure pump operatively connected to an electronic pressure controller/programmer for supplying fluid at high pressure to said inlet end, means such as a flow controller/restrictor for removing fluid from said outlet end, means such as a constant temperature oven for subjecting the fluid between said inlet and outlet ends to a temperature near (below, at or above) the critical temperature of the fluid, means for introducing a sample material into the fluid upstream from the inlet end for chromatographic analysis, such means preferably being an injector of the split injection type or optionally of the on-column type, and means for subjecting fluid to which a sample material has been added to detection by means of an ultraviolet detector, an on-column spectrofluorimeter, a mass spectrmeter, and the like. The method comprises carrying out the detection of material after subjecting it to supercritical fluid chromatography in a long stream of fluid, preferbly of capillary diameter, having an inlet end and an outlet end and which is subjected between said ends to near supercritical temperature and pressure for the fluid, the detection being carried on the fluid out near the outlet end by any suitable method including ultraviolet detection, spectrofluorimetric detection and mass spectrometric detection.

REFERENCES:
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