One-step test for aspartate aminotransferase

Details One-step test for aspartate aminotransferase One-step test for aspartate aminotransferase

1991-01-31

1998-11-10

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 16, 435 4, 435975, 435 74, 435184, 436 63, C12Q 148, C12Q 152, C12Q 100, G01N 3353

Patent

active

058342265

ABSTRACT:
Novel assay kits and methods are described for the detection and determination of the severity of disease conditions associated with elevated levels of aspartate aminotransferase (AST). The AST levels are determined by reaction of the AST with cysteine sulfinic acid (CSA) substrate in the presence of a sulfite-reactive dye compound that is substantially nonreactive with AST or CSA, such as a triarylmethine dye. Sulfite ion formed by the reaction of AST with CSA causes a calorimetric indication of AST that can be related to a predetermined stage of disease.

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