One-step free fatty acid determination method

Details One-step free fatty acid determination method One-step free fatty acid determination method

1993-11-24

1995-11-28

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435968, 436518, 436815, G01N 33533

Patent

active

054707145

ABSTRACT:
A one reaction step method for determining hydrophobic analytes, such as free hydrophobic analytes, comprising the steps of mixing a solution suspected of containing the hydrophobic analyte, e.g. free fatty acid, with a reagent comprising a fluorescently modified specific-binding protein for the hydrophobic analyte, detecting a fluorescence difference between the fluorescently modified specific-binding protein in the bound and unbound condition, and relating said fluorescence difference to the amount of arialyre in the solution is disclosed.

REFERENCES:
Richieri et al., A Fluorescently Labeled Intestinal Fatty Acid Binding Protein. Interactions with Fatty Acids and Its Use in Monitoring Free Fatty Acids. Journal of Biological Chemistry 267(33):23495-23501, 1992.

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