On-line enzymatic digestion in separation-detection methods

Details On-line enzymatic digestion in separation-detection methods On-line enzymatic digestion in separation-detection methods

2005-08-10

2008-09-09

Weber, Jon P (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S174000

Reexamination Certificate

active

07422866

ABSTRACT:
Apparatus and methods are disclosed for digesting a biopolymer in a medium. The medium is disposed adjacent a wall of a hollow element wherein at least a portion of the wall is porous. The medium is then exposed to digestion conditions to cleave the biopolymer into fragments. The fragments are permitted to permeate through the wall of the hollow element. In some embodiments a surface of a wall of the hollow element comprises a digestion agent. In some embodiments the hollow element is disposed within a hollow liner. For analysis and identification purposes, the fragments may be separated and/or detected.

REFERENCES:
patent: 4266026 (1981-05-01), Breslau
patent: 4448691 (1984-05-01), Davis
patent: 4455381 (1984-06-01), Magnusson et al.
patent: 4708800 (1987-11-01), Ichikawa et al.
patent: 5707516 (1998-01-01), Tomizawa et al.
patent: 5736036 (1998-04-01), Upchurch et al.
patent: 5885841 (1999-03-01), Higgs et al.
patent: 6022478 (2000-02-01), Baurmeister et al.
patent: 2003/0089605 (2003-05-01), Timperman
patent: 2003/0101866 (2003-06-01), Noack
patent: 2003/0104350 (2003-06-01), Bomberger et al.
patent: 2003/0104483 (2003-06-01), Davidson et al.
patent: 2003/0124606 (2003-07-01), Suckau et al.
patent: 2003/0129657 (2003-07-01), Decker et al.
patent: 2004/0171169 (2004-09-01), Kallury et al.
patent: 2005/0239152 (2005-10-01), Irth et al.
patent: 2006/0019399 (2006-01-01), Balgley et al.
patent: 2002057295 (2002-07-01), None
Trueb et al. Type VI Collagen is Composed of a 200 KD Subunit and Two 140 KD Subunits; The EMBO Journal, vol. 5, No. 11 (1986) pp. 2815-2819.
Davis, J.C. Kinetics Studies in a Continuous Steady State Hollow Fiber Membrane Enzyme Reactor; Biotechnology and Bioengineering, vol. 16 (1974) pp. 1113-1122.
Gao, Jun et al., “Integrated Microfluidic System Enabling Protein Digestion, Peptide Separation, and Protein Identification”, Anal. Chem. 2001, pp. 2648-2655, vol. 73, No. 11.
Vecchione, Gennaro et al., “A comprehensive on-line digestion-liquid chromatography/mass spectrometry/collision-induced dissociation mass spectrometry approach for the characterization of human fibrinogen”, Rapid Commun. Mass Spectrom. 2001, pp. 1383-1390, vol. 15.
EPO Communication dated Nov. 24, 2006 enclosing European Search Report, 4 pp., for EP Application No. 06253811 corresponding to U.S. Appl. No. 11/200,708.
Klein et al.; Journal of Proteome Research; vol. 1(1), 41-45 (2002); “A New Isoelectric Focusing Gel for Two-Dimensional Electrophoresis Constructed in Microporous Hollow Fiber Membranes”.
Choudhary et al.; Journal of Proteome Research; VBol. 2(1), 59-67 (2003); “Multiple Enzymatic Digestion for Enhanced Sequence Coverage of Proteins in Complex Proteomic Mixtures Using Capillary LC with Ion Trap MS/MS”.
Cooper et al.; Analytical Chemistry; vol. 75(5), 1067-1074 (2003); “Membrane-Based Nanoscale Proteolytic Reactor Enabling Protein Digestion, Peptide Separation, and Protein Identification Using Mass Spectrometry”.
Samskog et al.; Journal of Chromatography A; 998:83-91 (2003); “Miniaturized On-Line Proteolysis-Capillary Liquid Chromatography-Mass Spectrometry for Peptide Mapping of Lactate Dehydrogenase”.
Slysz et al. Rapid Commun. Mass Spectrom.; 17:1044-1050 (2003); “On-Column Digestion of Proteins in Aqueous-Organic Solvents”.

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