On-line coupling biochemical detection to continuous flow separa

Details On-line coupling biochemical detection to continuous flow separa On-line coupling biochemical detection to continuous flow separa

1996-04-19

2000-06-27

Stucker, Jeffrey

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

436538, 435 5, 435 71, 435 72, 435 79, 435DIG1, 435DIG2, 435DIG9, 435DIG14, 435DIG16, 435DIG17, 435DIG18, 435DIG22, 210656, G01N 33536, G01N 33537, G01N 3353, C12Q 170

Patent

active

060805900

ABSTRACT:
The present invention relates to an on-line detection method comprising the on-line coupling of a fractionation and a biochemical detection technique, which method comprises the addition of a controlled amount of an affinity molecule to the effluent of the fractionation step to react with analytes in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex. Further, the invention is directed to an on-line method for the screening of compounds on their binding capability to a known affinity molecule, which method comprises a fractionation step providing an effluent, the addition of a controlled amount of said affinity molecule to the effluent of the fractionation step and effecting a contact time sufficient to allow a reaction with or interaction between the compounds in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex.

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R.J. Miksicek: Interaction of naturally occuring nonesteroidal estrogens . . . : J. Steroid Biochem. Mol. Biol.: V. 49 (2-3): Abstract, 1994.
Irth, et al.: On-line immunochemical detection in liquid chroatography . . . : J Chrom. :633: pp 65-72, 1993.
Harlow, et al. Antibodies-a laboratory manual: pp. 555-583, 1988.

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