Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1992-03-06
1994-06-07
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447
Patent
active
053186800
ABSTRACT:
An on-column derivatization scheme where a liquid or solid labeling reagent is provided in a capillary to react chemically with a sample in order to label the sample. Electrophoretic separation may be carried out immediately thereafter to simplify the derivatization, separation and detection process. By localizing the labeling process to a small area within the capillary, fast kinetics and high reaction yield are achieved.
REFERENCES:
patent: 5045172 (1991-09-01), Guzman
patent: 5110431 (1992-05-01), Moring
Stephen L. Pentoney, Jr. et al., "On-Line Connector for Microcolumns: Application to the On-Column o-Phthaldialdehyde Derivativation of Amino Acids Separated by Capillary Zone Electrophoresis", Analytical Chemistry, vol. 60, No. 23 (Dec. 1, 1988) 2625-2629.
Richard M. Caprioli et al., "Coupling Cappillary zone Electrophoresis and Continuous-Flow Fast Atom Bombardment Mass Spectrometry for the Analysis of Peptide Mixtures", Journal of Chromatography, 480 (1989).
U. R. Tjaden, N. J. Reinhoud, and J. Vander Greef, "On-Line Derivatization In High Performance Capillary Electrophoresis", poster presentation at The Thirteenth Symposium on Column Liquid Chromatography, Stockholm, Sweden, Jun. 25-30, 1989.
"A Polymeric Reagent for Derivatization of Weak Nucleophiles in HPLC-UV", by Bourque and Krull, Journal of Chromatographic Science, 29:489-496, Nov. 1991.
"The Use of a Concentration Step to Collect Urinary Components Separated by Capillary Electrophoresis . . . ", by Guzman et al., Journ. of Liq. Chromatography 14(5):997-1015, 1991.
"The Use of On-Line Sample Concentration to Increase the Sensitivity of Capillary Electrophoresis", presented by Merion, et al., Mass. HPCE'91.
"Solid-Phase Reagent Containing the 3,5-Dinitrophenyl Tag for the Improved Derivatization of Chiral and Achiral Amines, . . . ", by Bourque et al., Journal of Chromatography, 537:123-152, 1991.
"On-Line Connector for Microcolumns: Application to the On-Column o-Phthaldialdehyde Derivatization of . . . ", by Pentoney, Jr. et al., Anal. Chem., 60:2625-2629, 1988.
"Capillary Isoelectric Focusing of Proteins in Uncoated Fused-Silica Capillaries Using Polymeric . . . ", by Mazzeo et al., Anal. Chem., 63:2852-2857, 1991.
"On-Line Derivatization in High Performance Capillary Electrophoresis", by Tjaden, et al., The Netherlands.
Patent Application Ser. No. 07/235,953 filed Aug. 24, 1988.
Colon Luis A.
Fishman Harvey A.
Shear Jason B.
Sweedler Jonathan V.
Zare Richard N.
Board of Trustees of the Leland Stanford Junior University
Niebling John
Starsiak Jr. John S.
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