Oligosaccharide sequencing

Details Oligosaccharide sequencing Oligosaccharide sequencing

1989-10-03

1992-03-31

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 22, 435101, C12Q 125

Patent

active

051007789

ABSTRACT:
A method of oligosaccharide sequencing in which the components are determined essentially simultaneously is disclosed which comprises a series of steps as follows:
A. Placing an identifying label on the reducing terminal residue of the oligosaccharide to be sequenced,
B. Dividing said oligosaccharide into a plurality of separate portions of known integer amounts,
C. Treating each said portion with a different reagent mix to thereby provide a series of reaction mixtures,
D. Pooling known integer amounts of the products from each separate reaction mixture to give a product pool,
E. Performing an analysis on said product pool which measures the molar proportions of the reaction products, and
F. Reconstructing or identifying the starting oligosaccharide from the molar prevalence of said reaction products.

REFERENCES:
patent: 4659659 (1987-04-01), Dwek et al.
patent: 4751084 (1988-06-01), Feder et al.
Harada, Anal-Biochem. 164,374-381 (1987).
Parekh et al., Nature 316,452-457 (1985).
Kobata, Anal. Biochem. 100,1-14 (1979).

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