Oligonucleotides useful as adaptors in DNA cloning, adapted DNA

Details Oligonucleotides useful as adaptors in DNA cloning, adapted DNA Oligonucleotides useful as adaptors in DNA cloning, adapted DNA

1977-10-19

1982-03-23

Hazel, Blondel

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, C07H 2104

Patent

active

043213650

ABSTRACT:
Synthetic oligonucleotides have been designed and prepared which are useful in the molecular cloning of a variety of DNA molecules. By means of such oligonucleotides, genetic informational material, e.g., DNA, can be joined to a cloning vehicle and transferred into host cells by transformation. Additionally, a method for determining whether genetic informational material has been transferred into transformed host cells has been developed.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Marians et al., Nature, vol. 263, pp. 744-748 (1976).
Heyneker et al., Nature, vol. 263, pp. 748-752 (1976).
Scheller et al., Science, vol. 196, pp. 177-180 (1977).
Bahl et al., Gene 1, 81-92 (1976).
Pribnow, Proc. Nat. Acad. Sci., vol. 72, pp. 784-788, 1975.
Polisky et al., Proc. Nat. Acad. Sci., vol. 73, pp. 3900-3905 (1976).
Itakura et al., Science, vol. 198, pp. 1056-1066 (1977).

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