Oligonucleotides, methods and kits for amplifying and detecting

Details Oligonucleotides, methods and kits for amplifying and detecting Oligonucleotides, methods and kits for amplifying and detecting

1997-04-18

1998-12-29

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 170

Patent

active

058539816

ABSTRACT:
The present invention relates to two primers for amplifying a cytomegalovirus (CMV) nucleic acid suitable for a nucleic acid sequence-based amplification (NASBA) using a DNA-dependent RNA polymerase, a first primer containing a promoter sequence and a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:1; a second primer containing a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:2, NO:3 or NO:4; a detecting probe and/or a capturing probe containing a nucleic acid sequence consisting of at least continuous fifteen bases selected from the nucleic acid sequence(s) of SEQ ID NO:5, NO:6 and/or NO:7 wherein said sequence is modified if necessary; a reagent kit for detecting a CMV containing the above-mentioned primers and probes; and a nucleic acid sequence-based amplification (NASBA) using said primers. The advantages of the present invention are that CMV can be detected in an easy, rapid and specific manner with a high sensitivity.

REFERENCES:
patent: 5409818 (1995-04-01), Davey et al.
Kondo et al, "Human cytomegalovirus latent infection of granulocyte-macrophage progenitors", Proc. Natl. Acad. Sci. USA, vol. 91, pp. 11879-11883, Dec. 1994.
Sooknanan et al, "NASBA, A detection and amplification system uniquely suited for RNA", Biotechnology, vol. 13, pp. 563-564, Jun. 1995.
Vandamme et al, "Detection of HIV-1 RNA in plasma and serum samples using the NASBA amplification system compared to RNA-PCR", J. of Virol. Meth., vol. 52, pp. 121-132, 1995.

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