Oligonucleotides having random riplets

Details Oligonucleotides having random riplets Oligonucleotides having random riplets

1995-06-05

1998-09-15

Campbell, Eggerton A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, C07H 2102, C07H 2104

Patent

active

058080228

ABSTRACT:
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.

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Blake et al., "Tuplet Analysis of Sequences." Federation Proceedings-Abstracts. Index #2958 (1983).
Cwirla et al., "Peptides on phage: A vast library of peptides for identifying ligands." Proc. Natl. Acad. Sci. USA 87:6378-6382 (1990).
Devlin et al., "Random Peptide Libraries: A Source of Specific Protein Binding Molecules", Science 249:404-406 (1990).
Scott, Jamie K. and Smith, George P., "Searching for Peptide Ligands with an Epitope Library", Science 249:396-390 (1990).
Lehtovaara et al, Protein Engineering 2: 63-68, 1988.
Oliphant et al, Gene 44:177-183 (1986).

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