Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-11-01
1998-03-31
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435912, 536243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
057337241
ABSTRACT:
The invention relates to oligonucleotides which are suitable for use as nucleic acid probes or as primers for the detection of Enterobacteriaceae, in particular of pathogenic Enterobacteriaceae, such as, for example, Salmonella sp. The oligonucieotides according to the invention are selected from the salmolysin-like DNA region and possess a sequence according to one of the formulae IIIa to IIIh, or a corresponding complementary sequence:
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Libby et al, "A cytolysin encoded by Salmonella is required for survival within macrophages", Proc. Natl. Acad. Sci. 91(2):489-493, Jan. 1994.
Libby et al, "Cloning and characterization of a cytotoxin gene from Salmonella typhimurium", Res. Microbiol. 141:775-783, 1990.
Stratagene catalog, p. 39, 1988.
"Molecular Hybridization of Immobilized Nucleic Acids: Theoretical Concepts and Practical Considerations", Wahl et al., Methods in Enzymology, vol. 152, 1987, pp. 399 through 407.
"Hybridization of DNA or RNA Immobilized on Filters to Radioactive Probes", Maniatis et al., Molecular Cloning, 1982, pp. 324-325.
"Efficient isolation of genes by using antibody probes", Young et al., Proc. Natl. Acad. Sci. (USA), vol. 80, Mar. 1983, pp. 1194-1198.
"Use of the DNA polymerase chain reaction for homology probing: Isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species", Gould et al., Proc. Natl. Acad. Sci. (USA), vol. 86, Mar. 1989, pp. 1934-1938.
"Use of synthetic oligonucleotides as hybridization probes: Isolation of clones cDNA sequences for human .beta..sub.2 -microglobulin*", Suggs et al., Proc. Natl. Acad. Sci., vol. 78, No. 11, Nov. 1981, pp. 6613-6617.
"Purification of .alpha.-hemolysin from an overproducing E. coli strain", Gonzales-Carrero et al., Mol. Gen. Genet, vol. 199, 1985, pp. 106-110.
Bubert Andreas
Goebel Werner
Goetz Monika
Ludwig Albrecht
Neumann Siegfried
Elliott George C.
Fredman Jeffrey
Merck Patent Gesellschaft mit beschrankter Haftung
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