Oligonucleotides for detecting enteric hemorrhagic E.coli and de

Details Oligonucleotides for detecting enteric hemorrhagic E.coli and de Oligonucleotides for detecting enteric hemorrhagic E.coli and de

1999-05-19

2000-12-26

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2432, 536 2433, 536 266, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

061657247

ABSTRACT:
The object of the present invention is to provide a simple, rapid and highly sensitive method of examining EHEC or VTEC in the examination of food poisoning and diarrhea. In the present invention, the oligonucleotides of SEQ ID NOS: 1-9 hybridizing selectively with a Vero toxin gene from EHEC (or VTEC) or with an O antigen-synthesizing region gene from pathogenic E. coli O157 are prepared and used as primers for gene amplification. By this method, bacteria producing O157 as one of the pathogenic factors in these bacteria and E. coli capable of producing Vero toxin can be selectively detected.

REFERENCES:
patent: 5795717 (1998-08-01), Nakayama et al.
Franke et al.,"Clonal Relatedness of Shiga-like Toxin-producing Escherechia coli o101 strains of Human and Porcine Origin", Journal of Clinical Microbiology, vol. 33 (12), pp. 3174-3178, Sep. 1995.

Affiliated with

Also associated with

Rating

Oligonucleotides for detecting enteric hemorrhagic E.coli and de does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oligonucleotides for detecting enteric hemorrhagic E.coli and de, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotides for detecting enteric hemorrhagic E.coli and de will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-993195