Oligonucleotides for detecting bacteria and detection method usi

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 231, 536 237, 536 2433, 435 6, 435 912, 435848, 935 8, 935 77, 935 78, C07H 2104, C12Q 168, C12N 100, C12N 1500

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055257186

ABSTRACT:
Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

REFERENCES:
Nishibuchi et al., Infect. Immun. 57(9):2691-2697, 1989.
Nishibuchi et al. Mol Microbiol 4(1)87-100, 1990.
M. J. Bentley et al., "Nucleotide Sequence of the Type A Staphylococcal Enterotoxin Gene," The Journal of Bacteriology, vol. 170, No. 1, issued Jan. 1988, pp. 34-41.

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