Oligonucleotides containing an antisense sequence stabilized...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Reexamination Certificate

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07022832

ABSTRACT:
Oligonucleotides capable of modifying or inhibiting in vivo or in vitro expression of a target gene wherein the oligonucleotide has an antisense sequence, at least one secondary structure, and optionally a supplementary nucleotide sequence located at one and/or both ends of the antisense sequence and wherein the secondary structure disintegrates upon attachment of the oligonucleotide to a target nucleic acid; a pharmaceutical composition containing such an oligonucleotide as an active ingredient; and a method of treatment using such an oligonucleotide.

REFERENCES:
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patent: WO 94/12633 (1994-06-01), None
patent: WO 94/23026 (1994-10-01), None
patent: WO 95/29241 (1995-11-01), None
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Abe, T. et al., “Specific inhibition of influenza virus RNA polymerase and nucleoprotein gene expression by circular dumbdell RNA/DNA chimeric oligonucleotides containing antisense phosphodiester oligonucleotides,” Febs Lett 425(1), Mar. 20, 1998, pp. 91-96.
Barker RH Jr. et al, Plasmodium falciparum: Effect of Chemical Structure on Efficacy and Specificity of Antisense Oligonucleotides against Malaria in Vitro, Experimental Parasitology 88, Jan. 1998, pp. 51-59.
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Tanaka, Kazuhiro et al., EWS-Flil Antisense Oligodeoxynucleotide Inhibits Proliferation of Human Ewings's Sarcoma and Primitive Neurectodermal Tumor Cells, Journal of Clinical Investigation, vol. 99, No. 2, 1997, pp. 239-247.
Gottikh, M.B. et al, “α-β Chimeric Oligonucleotides Form a New Stable “Snail-like”Structure,”, J. Am. Chem. Soc., 1996, 118, pp. 2126-2130.
Tang, J. et al, Self-stabilized antisense oligodexynucleotide phosphorothioates: properties and anti-HIV activity, Nucl. Acids Research, vol. 21, No. 11, 1993, pp. 2729-2735.

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