Oligonucleotides and nucleic acids for detection of ureaplasma u

Details Oligonucleotides and nucleic acids for detection of ureaplasma u Oligonucleotides and nucleic acids for detection of ureaplasma u

1994-04-18

1998-03-17

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 77, 935 78, C12Q 168, C12P 1934

Patent

active

057285225

ABSTRACT:
The present invention is directed to a rapid and sensitive method for detecting Ureaplasma urealyticum using U. urealyticum-specific probes and oligonucleotides. In particular a target sequence can be amplified by in vitro nucleic acid amplification techniques or directly detected by nucleic acid hybridization using the subject probes and oligonucleotides. U. urealyticum-specific nucleic acids which do not recognize or hybridize to genomic nucleic acid of Mycoplasma species are also provided.

REFERENCES:
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Blanchard, A. (1990) Ureaplasma urealyticumUrease Genes; Use of a UGA Tryptophan Codon, Molecular Microbiology 4(4):669-676.
Roberts et al. (1987) DNA Probes for the Detection of Mycoplasmas in Genital Specimens, Israel Journal of Medical Sciences 23:618--620.
Gonzales et al. (1991) A Non-isotopic DNA Probe for the Specific Detection of Ureaplasma, Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 91, p.81.
Hammond et al. (1991) Biotype Specific Probes for Ureaplasma urealyticum, Abstr. Gen. Meet.Am. Soc. Microbiol., vol. 91, p. 81.
Wright et al. (1990) The Polymerase Chain Reaction: Miracle or Mirage? A Critical Review of its Uses and Limitations in Diagnosis and Research, Journal of Pathology 162:99-117.

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