Oligonucleotides and methods for the detection of Neisseria gono

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2432, 536 2433, 935 78, C12Q 168, C12P 1934, C07H 2104

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054533554

ABSTRACT:
The present invention relates to oligonucleotide probes and primers useful in detecting Neisseria gonorrhoeae e.g. by the polymerase chain reaction. The present invention is also directed to methods for detecting Neisseria gonorrhoeae by the polymerase chain reaction. The probes and primers are specific for the pilin gene.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5162199 (1992-11-01), Stern et al.
Preliminary Evaluation of the Ligase Chain Reaction for Specific Detection of Neisseria gonorrhoeae Birkenmeyer, et al Journal of Clinical Microbiology, pp. 3089-3094, 1992.
Silent pilin genes of Neisseria gonorrhoeae MS11 and the occurrence of related hypervariant sequences among other gonococcal isolates Haas, et al Molecular Microbiology, vol. 6, No. 2, pp. 197-208, 1992.
L-pilin variants of Neisseria gonorrhoeae MS11 Manning, et al Molecular Microbiology, vol. 5, No. 4, pp. 917-926, 1991.
Structural analysis of the pilE region of Neisseria gonorrhoeae P9 Perry, et al Gene, vol. 60, pp. 85-92, 1987.
Meyer et al., PNAS 81, 6110-6114 (1984).

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