Oligonucleotides and methods for the detection of Chlamydia trac

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 9151, 536 2433, C12Q 168, C12P 1934, C07H 2104

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active

057562989

ABSTRACT:
The present invention relates to oligonucleotide probes useful in detecting, e.g. by hybridization or the ligase chain reaction, Chlamydia trachomatis DNA in the presence of other related DNA. The present invention is also directed to methods of detecting Chlamydia trachomatis organisms in a sample using the ligase chain reaction.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5232829 (1993-08-01), Longiaru et al.
Hatt, C., et al., "Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar LI. Evidence for involvement in DNA replication", Nucleic Acids Research, 16(9):4053-4067 (1988).
Baehr, W., et al., "Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes", Proc. Natl. Acad. Sci., 85:4000-4004 (1988).
Stephens, R.S., et al., "Diversity of Chlamydia trachomatis Major Outer Membrane Protein Genes", Journal of Bacteriology, 169(9):3879-3885 (1987).
Dille, B.J., et al., "Amplification of Chlamydia trachomatis DNA by Ligase Chain Reaction", Journal of Clinical Microbiology, 31(3):729-731 (1993).
Pickett, M.A., et al., "Complete nucleotide sequence of the major outer membrane protein gene from Chlamydia trachomatis serovar L1", FEMS Microbiology Letters, 42:185-190 (1987).
Dayada, C., et al., "Complete sequence of the major outer membrane protein-encoding gene of Chlamydia trachomatis serovar Da*", Gene, 120:129-130 (1992).
Zhang, Y-X, et al., "The nucleotide sequence of major outer membrane protein gene of Chlamydia trachomatis serovar F", Nucleic Acids Research, 18(4):1061 (1989).

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