Oligonucleotide which can be used as primer in a method of ampli

Details Oligonucleotide which can be used as primer in a method of ampli Oligonucleotide which can be used as primer in a method of ampli

1995-10-27

1999-02-23

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 2433, C12P 1934

Patent

active

058742603

ABSTRACT:
Oligonucleotide comprising, from 5' to 3', a first portion comprising an upstream segment and a downstream segment forming a stem-loop structure, and a second portion not capable of self-pairing, in which the first portion comprises at least one blocking agent, capable of blocking the replication, by a polymerase, of the said oligonucleotide, such that at least one portion of the downstream segment is replicated and such that the upstream segment is replicated neither totally nor partially. This oligonucleotide can be used as primer in a process for the cyclic amplification of a target sequence of a nucleic acid.

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patent: 5369003 (1994-11-01), Reischl et al.
patent: 5411875 (1995-05-01), Jones
patent: 5474916 (1995-12-01), Reischl et al.
G. Caetano-Anolies, "DNA Amplification Fingerprinting Using Arbitrary Mini-hairpin Oligonucleotide Primers",Bio/Technology, vol. 12, Jun. 1994, pp. 619-623.
G. Krupp et al., "Simplified in vitro synthesis of mutated RNA molecules", Federation of European Biochemical Societies, vol. 212, Feb. 1987, pp. 271-275.
W. Carpenter et al., "A Transcriptionally Amplified DNA Probe Assay with Ligatable Probes and Immunochemical Detection", Clin. Chem. vol. 39, No. 9, 1993, pp. 1934-1938.

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