Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-10-27
1999-02-23
Houtteman, Scott W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
536 2433, C12P 1934
Patent
active
058742603
ABSTRACT:
Oligonucleotide comprising, from 5' to 3', a first portion comprising an upstream segment and a downstream segment forming a stem-loop structure, and a second portion not capable of self-pairing, in which the first portion comprises at least one blocking agent, capable of blocking the replication, by a polymerase, of the said oligonucleotide, such that at least one portion of the downstream segment is replicated and such that the upstream segment is replicated neither totally nor partially. This oligonucleotide can be used as primer in a process for the cyclic amplification of a target sequence of a nucleic acid.
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G. Caetano-Anolies, "DNA Amplification Fingerprinting Using Arbitrary Mini-hairpin Oligonucleotide Primers",Bio/Technology, vol. 12, Jun. 1994, pp. 619-623.
G. Krupp et al., "Simplified in vitro synthesis of mutated RNA molecules", Federation of European Biochemical Societies, vol. 212, Feb. 1987, pp. 271-275.
W. Carpenter et al., "A Transcriptionally Amplified DNA Probe Assay with Ligatable Probes and Immunochemical Detection", Clin. Chem. vol. 39, No. 9, 1993, pp. 1934-1938.
Cleuziat Philippe
Guillou-Bonnici Francoise
Levasseur Pierre
Mallet Francois
Bio Merieux
Houtteman Scott W.
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