Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-22
1997-12-23
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
057006423
ABSTRACT:
The present invention provides modified oligonucleotide primers that (i) are designed for attachment to a solid support in a manner that does not block the ability to extend the primer from its 3' end, and (ii) incorporate a clearable moiety so that a 3' portion of the primer (linked to an extension product) can be released from an immobilized 5' portion. Upon selective cleavage of the cleavable site, a large portion of the primer fragment remains affixed to the solid support. This enables the release of primer extension products that contain about five or fewer base pairs of the primer sequence, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
REFERENCES:
patent: 4775619 (1988-10-01), Urdea
patent: 4818681 (1989-04-01), Dattagupta
patent: 5106585 (1992-04-01), Minami et al.
patent: 5118605 (1992-06-01), Urdea
patent: 5288644 (1994-02-01), Beavis et al.
patent: 5405746 (1995-04-01), Uhlen
patent: 5547835 (1996-08-01), Koster
Bahr, U., et al., "Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry," Fresenius J. Anal. Chem. 348:783-791 (1994).
Barinaga, M., "Protein Chemists Gain a New Analytical Tool," Science 246:32-33 (1989).
Brummel, C.L., et al., "A Mass Spectrometric Solution to the Address Problem of Combinatorial Libraries," Science 264:399 (1994).
Fitzgerald, M.C., et al., "The Analysis of Mock DNA Sequencing Reactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry,"Rapid Comm. in Mass Spectrom. 7:895-897 (1993).
Goldkorn, T., and Prockop, D.J., "A Simple and Efficient Enzymatic Method for Covalent Attachment of DNA to Cellulose: Application for Hybridization-Restriction Analysis and for In Vivo Synthesis of DNA Probes," Nuc. Acids. Res. 14:9171 (1986).
Hettich, R.L., and Buchanan, M.V., "Determination of Oligonucleotide Sequences and Modifications by Laser Desorption Fourier Transform Mass Spectrometry," Abst. Pap. Am. Chem. Soc. 200:1-2, Abstract 105 (1990).
Hillenkamp, F., and Karas, M., "Matrix Assisted UV-Laser Desorption/Ionization: A New Approach to Mass Spectrometry of Large Biomolecules" in Biological Mass Spectrometry (Burlingame, A.L., and McCloskey, J.A., Eds.), Elsevier Science Publishers B.V., Amerstam, Holland (1988).
Hillenkamp, F., and Karas, M., "Laser Desorption Ionization of Proteins with Molecular Masses Exceeding 10,000 Daltons," Anal. Chem. 60:2299-2301 (1988).
Hillenkamp, F., and Karas, M., "Matrix Laser Desorption of Very Large Organic Molecules" in Mass Spectrometry of Large Non-Volatile Molecules for Marine Organic Chemistry (Hilf, E.R., and Tuszynski, W., Eds.) World Scientific Publishers, Singapore, (1990).
Lay, Jr., J.O., et al., "Detection and Characterization of DNA Adducts at the Femtomole Level by Desorption Ionization Mass Spectrometry," Environ. Health Perspect. 99:191-193 (1993).
Mag, M., et al., "Synthesis and Selective Cleavage of an Oligodeoxynucleotide Containing a Bridged Internucleotide 5'-Phosphorothioate Linkage," Nuc. Acids Res. 19(7):1437-1441 (1991).
Maskos, U., and Southern, E.M., "Oligonucleotide Hybridisations on Glass Supports: A Novel Linker for Oligonucleotide Synthesis and Hybridisation Properties of Oligonucleotides Synthesis In Situ," Nuc. Acids Res. 20(7):1679-1684 (1992).
Nadji, S., et al., "Photochemically and Photoenzymatically Cleavable DNA," J. Am. Chem. 114:9266-9269 (1992).
Nordhoff, E., et al., "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Nucleic Acids with Wavelengths in the Ultraviolet and Infrared," Rapid Comm. in Mass Spectrom. 6:771-776 (1992).
Parr, G.R., et al., "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Synthetic Oligodeoxyribonucleotides," Rapid Comm. in Mass Spectrom. 6:369-372 (1992).
Stemmler, E.A., et al., "Matrix-Assisted Laser Desorption/Ionization Fourier-Transform Mass Spectrometry of Oligodeoxyribonucleotides," Rapid Comm. in Mass Spectrom. 7:828-836 (1993).
Tang, K., et al., "Mass Spectrometry of Laser-Desorbed Oligonucleotides," Rapid Comm. in Mass Spectrom. 6:365-368 (1992).
Tang, K., et al., "Laser Mass Spectrometry of Oligonucleotides with Isomer Matrices," Rapid Comm. in Mass Spectrom. 7:435-439 (1993).
Tong, X., and Smith, L.M., "Solid Phase Purification in Automated DNA Sequencing," J. DNA Seq. and Mapping 4:151-162 (1993).
Wu, K.J., et al., "Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry of Oligonucleotides Using 3-Hydroxypicolinic Acid as an Ultraviolet-Sensitive Matrix," Rapid Comm. in Mass Spectrom. 7:142-146 (1993).
Wu, K.J., et al., "Time-of-Fight Mass Spectrometry of Underivatized Single-Stranded DNA Oligomers by Matrix-Assisted Laser Desorption," Anal. Chem. 66:1637-1645 (1994).
Youngquist, R.S., et al., "Matrix-Assisted Laser Desorption Ionization for Rapid Determination of the Sequences of Biologically Active Peptides Isolated from Support-Bound Combinatorial Peptide Libraries," Rapid Comm. in Mass Spectrom. 8:77-81 (1994).
Silveira, M.H., and L.E. Orgel, "PCR with detachable primers," Nucleic Acids Research 23(6): 1083-1084 (1995).
Frohman, M.A., "Cloning PCR Products," Chapter 2 in The Polymerase Chain Reaction (Mullis, K.B., et al., eds., Birkhauser Boston, 1994, pp. 14-37).
Szybalski, W., "Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties," Gene 40: 169-173 (1985).
Nakamaye, K., et al., Direct Sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside a-thiotriphosphates, Nuc. Acids Res. 16(21):9947-9959, 1988.
Becker Christopher Hank
Monforte Joseph Albert
Pollart Daniel Joseph
Shaler Thomas Andrew
Campbell Eggerton A.
Evans Susan T.
Fabian Gary R.
SRI - International
LandOfFree
Oligonucleotide sizing using immobilized cleavable primers does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Oligonucleotide sizing using immobilized cleavable primers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide sizing using immobilized cleavable primers will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1801727