Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1998-10-20
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436 94, 436501, C12Q 168
Patent
active
058244751
ABSTRACT:
The invention features a method for assaying the ability of an oligonucleotide to form a hybrid with a target nucleic acid sequence. The featured assay comprises the following steps: (a) contacting a test sample containing a target nucleic acid sequence with an oligonucleotide; (b) treating the test sample with a duplex-altering agent; (c) contacting the treated test sample with a labeled probe under stringent hybridization conditions, wherein the probe can hybridize to non-altered target nucleic acid sequence such that the probe label is protected from subsequent chemical inactivation but the probe cannot hybridize to altered target nucleic acid sequence in a manner which would protect the probe label from subsequent chemical inactivation; and (d) measuring the hybridization of the probe to non-altered target nucleic acid sequence by measuring the amount of protected label. Also described is an assay to measure the ability of a ribozyme to cleave a target nucleic acid sequence, and an assay to measure the ability of an agent to alter nucleic acid.
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Kacian Daniel L.
Nelson Norman C.
Velarde, Jr. Jorge
Gen-Probe Incorporated
Horlick Kenneth R.
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