Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1992-08-28
1995-11-07
Parr, Margarat
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2433, 435 6, 435 912, 935 6, 935 78, C07H 2104, C12Q 168
Patent
active
054649455
ABSTRACT:
The present invention provides methods and reagents for the estimation of the quantity of human DNA contained in a sample. Immobilized sample DNA is hybridized to a biotinylated oligonucleotide probe that hybridizes to a human genomic or mitochondria DNA sequence. The subsequent binding of streptavidin-horseradish peroxidase to the bound probe allows for chemiluminescent detection using a luminol-based reagent and X-ray film. In addition, the present invention provides methods and reagents to assess the quality of DNA contained in a sample. The sample is first size fractionated by agarose gel electrophoresis, and then immobilized, hybridized to a biotinylated oligonucleotide probe, and detected using the chemiluminescent method as used in the quantity estimation methods of the present invention.
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Reynolds Rebecca L.
Walsh P. Sean
Gould George M.
Hoffmann-La Roche Inc.
Myers Carla
Parr Margarat
Sias Stacey R.
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