Oligonucleotide probe conjugated to a purified hydrophilic alkal

Details Oligonucleotide probe conjugated to a purified hydrophilic alkal Oligonucleotide probe conjugated to a purified hydrophilic alkal

1995-06-07

1998-07-14

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 77, 935 78, C12Q 168, C07H 2104, C07H 2102

Patent

active

057802270

ABSTRACT:
A method of preparing a homogeneous alkaline phosphatase-oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described.

REFERENCES:
patent: 4868105 (1989-09-01), Urdea et al.
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 5124246 (1992-06-01), Urdea et al.
patent: 5254469 (1993-10-01), Warren, III et al.
Jablonski et al. Nucl;eic Acids Research 14 (15) : 6115-6128 (1986).
Bublitz et al., "Heterogeneity of glycosylphosphatidylinositol-anchored alkaline phosphatase of calf intestine" Eur. J. Biochem. 217:199-207 (1993).
Schaap et al., "Chemical and enzymatic triggering of 1, 2-dioxetanes. 3: Alkaline phosphatase-catalyzed chemiluminescence from an aryl phosphate-substituted dioxetane" Tet. Lett. 27:1159-1162 (1987).
Enrico Davini et al., "Alkaline Phosphatase Inhibitors as Labels of DNA Probes," Elsevier Science Pub. Co., Inc. GATA 9(2):39-47, (1992).
Renz et al., Nucleic Acids Research 12(8): 3435-3444 (1984).

Affiliated with

Also associated with

Rating

Oligonucleotide probe conjugated to a purified hydrophilic alkal does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oligonucleotide probe conjugated to a purified hydrophilic alkal, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide probe conjugated to a purified hydrophilic alkal will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1880475