Oligonucleotide probe and primers specific to bovine or porcine

Details Oligonucleotide probe and primers specific to bovine or porcine Oligonucleotide probe and primers specific to bovine or porcine

1994-02-14

1997-01-21

Zitomer, Stephanie W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 912, 4351723, 435810, 435501, 536 231, 536 241, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C07H 2104, C12Q 168

Patent

active

055960898

ABSTRACT:
The present invention relates to novel bovine (SEQ ID NO:1) and porcine (SEQ ID NO:2) genomic sequences for the SRY gene along with oligonucleotide primers. The present invention also relates to a method of sexing bovine or porcine tissue by descriminating PCR products obtained by amplification of specific DNA or cDNA sequences of bovine or porcine tissue which is used as a DNA template and wherein two pairs of DNA primers are used for the PCR. The present invention also relates to a method for the genetic manipulation or selection of sexual phenotype in domesticated animals, which comprises using transgenes composed of SRY sequences to cause and control the expression of genetic ablation sequences and genetic switching sequences in undifferentiated and developing gonadal tissues of both XX and XY animals.

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