Oligonucleotide probe

Organic compounds -- part of the class 532-570 series – Organic compounds – Halogen containing

Reexamination Certificate

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C570S157000

Reexamination Certificate

active

07491857

ABSTRACT:
An aminated oligonucleotide probe is provided, in which the amino group possesses improved reactivity. The present invention relates to an oligonucleotide probe, which is represented by general formula 1:(wherein R1is a hydrogen atom or a protecting group for an amino group, R2and R3are each independently a divalent organic group, and A is an oligonucleotide).

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Muthiah Manoharan, et al, “N-(2-cyanoethoxycarbonyloxy) succinimide: a new reagent for protection of amino groups in oligonucleotides”, Journal of Organic Chemistry, 1999, vol. 64, No. 17, pp. 6468-6472.
Uwe Maskos, et al, “Parallel Analysis of Oligodeoxyribonucleotide (oligonucleotide) Interactions. I. Analysis of Factors Influencing Oligonucleotide Duplex Formation”, Nucleic Acids Research, vol. 20, No. 7, 1991, pp. 1675-1678.
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Sudhir Agrawal, et al, “Efficient Methods for Attaching Non-Radioactive Labels to the 5′ Ends of Synthetic Oligodeoxyribonucleotides”, Nucleic Acids Research, vol. 14, No. 15, 1986, pp. 6227-6245.

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