Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1996-10-28
1998-11-17
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, 435810, 536 2432, 536 2433, 935 8, 935 11, 935 77, 935 78, C12Q 170, C12Q 168, C12P 2934, C07H 2104
Patent
active
058374426
ABSTRACT:
The present invention provides improved primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from hepatitis C virus (HCV). The primers and amplification methods of the invention enable the detection of HCV with greatly increased sensitivity.
REFERENCES:
patent: 5527669 (1996-06-01), Resnick et al.
Choo et al., Mar., 1991, "Genetic Organization and Diversity of the Hepatitis C Virus" Proc. Natl. Acad. Sci. USA 88:2451-2455.
Young et al., Apr., 1993, "Detection of Hepatitis C Virus RNA by a Combined Reverse Transcription-Polymerase Chain Reaction Assay" J. Clinical Microbiology 31(4):882-886.
Young et al., Mar., 1995, "Detection of Hepatitis C Virus RNA by a Combined Reverse Transcription PCR Assay: Comparision with Nested Amplification and Antibody Testing" J. Clinical Microbiology 33(3):654-657.
Simmonds, Feb., 1995, "Variability of Hepatitic C Virus" Hepatology 21(2):570-583.
Kleter et al, Journal of Medical Virology (1995) 47: 35-42.
Stuyver et al, Journal of General Virology (1993) 74: 1093-1102.
Schlauder et al, Journal of Virological Methods (1992) 37: 189-200.
Johnston George W.
Myers Carla J.
Petry Doug
Roche Molecular Systems Inc.
Tramaloni Dennis P.
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