Oligonucleotide population preparation

Details Oligonucleotide population preparation Oligonucleotide population preparation

1996-01-16

1997-08-19

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 242, 536 231, 435 913, C12Q 168, C07H 2104

Patent

active

056587368

ABSTRACT:
Methods are disclosed for producing a sequence tag from a polynucleotide. In preferred embodiments, the method comprises (a) digesting the polynucleotide with a type II restriction enzyme to produce a first cohesive end; (b) providing a promoter-linker cassette comprising an RNA polymerase promoter, a restriction site for a type IIS restriction enzyme and a second cohesive end which is complementary to the first cohesive end produced by the type II restriction enzyme; (c) ligating the digested polynucleotide to the cassette by joining the first cohesive end and the second cohesive end; (d) digesting the ligated product of step (c) with the type Iis restriction enzyme; and (e) transcribing the resulting construct from the promoter thereby producing a sequence tagged polyribonucleotide.

REFERENCES:
patent: 4942124 (1990-07-01), Church
patent: 5149625 (1992-09-01), Church et al.
patent: 5173410 (1992-12-01), Ahlquist
Velculescu et al, Science 270:484-487 (1995).
Szybalski et al., Gene 100:13-26 (1991).
Jarvik et al. (1996) BioTechniques 20:896-904.
Church et al. (1988) Science 240:185-240.

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