Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1992-03-17
1994-11-08
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 536 2531, 536 2762, C07H 2100, C12Q 168
Patent
active
053628667
ABSTRACT:
A versatile polymeric support system for the synthesis of oligonucleotides is provided featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolating and detecting complementary polynucleic acids.
REFERENCES:
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4959463 (1990-09-01), Froehler et al.
Kochetkov et al., Organic Chemistry of Nucleic Acids, Pat. A, Plenum Press, New York, N.Y., 1971, pp. 48-49.
Brown Johnnie R.
Crane L. Eric
Molecular Biosystems, Inc.
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