Oligonucleotide pairs for multiplexed binding assays

Details Oligonucleotide pairs for multiplexed binding assays Oligonucleotide pairs for multiplexed binding assays

2007-03-27

2007-03-27

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100

Reexamination Certificate

active

10419020

ABSTRACT:
The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

REFERENCES:
patent: 5604097 (1997-02-01), Brenner
patent: 5635400 (1997-06-01), Brenner
patent: 5654413 (1997-08-01), Brenner
patent: 5807683 (1998-09-01), Brenner
patent: 5846719 (1998-12-01), Brenner et al.
patent: 5863722 (1999-01-01), Brenner
patent: 6140489 (2000-10-01), Brenner
patent: 6146833 (2000-11-01), Milton
patent: 6172214 (2001-01-01), Brenner
patent: 6172218 (2001-01-01), Brënner
patent: 6235475 (2001-05-01), Brenner et al.
patent: 6352828 (2002-03-01), Brenner
patent: 2005/0026164 (2005-02-01), Zhou
patent: WO 96/06948 (1996-03-01), None
patent: WO 02/16649 (2002-02-01), None
Agrawal, Sudhir et al.,Site Specific Functionalization of Oligonucleotidesfor Attaching Two Different Reporter Groups, Nucleic Acids Research, 18:5419-5423 (1990).
Matson, Robert et al.,Biopolymer Synthesis on Polypropylene Supports: Oligonucleotide Arrays, Analytical; Biochemistry 224, 110-116 (1995).
Ozaki, Hiroaki et al.,The Estimation of Distances Between Specific Backbone-Labeled Sites in DNA Using Fluorescence Resonance Energy Transfer, Nucleic Acids Research, 20:5205-5214 (1992).
Uhlmann, Eugen,Antisense Oligonucleotides: A New Therapeutic Principle, Chemical Reviews, 90:543-584 (1990).
Oligonucleotide Properties Calculator, http://www.basic.nwu.edu/biotools/oligocalc.html.
Ben-Dor, A. et al., “Universal DNA Tag Systems: A Combinatorial Design Scheme,”Journal of Computational Biology,7(3/4):503-519 (2000).
Gerry, Norman P. et al., Universal DNA Microarray method for Multiplex Detection of Low Abundance Point Mutations,Journal of Molecular Biology, 292(2):251-262 (Sep. 17, 1999).
Shoemaker, D.D. et al., “Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy,”Nature Genetics, 14(4):451,452,455 (Dec. 1, 1996).

Affiliated with

Also associated with

Rating

Oligonucleotide pairs for multiplexed binding assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oligonucleotide pairs for multiplexed binding assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide pairs for multiplexed binding assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3805813