Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-06
1997-05-20
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 536 231, 536 245, 536 254, 536 243, C12Q 168, C12P 1934, C07H 2104
Patent
active
056311354
ABSTRACT:
Modified oligonucleotides 3'-NHP(O)(O.sup.-)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.
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Chen Jer-Kang
Gryaznov Sergei M.
Schultz Ronald G.
Chambers Jasemine C.
Lynx Therapeutics, Inc.
Priebe Scott D.
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