Oligonucleotide libraries useful for producing primers

Details Oligonucleotide libraries useful for producing primers Oligonucleotide libraries useful for producing primers

1996-02-20

1999-01-12

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 243, 536 2433, 536 253, C12P 1934, C07H 2104

Patent

active

058587314

ABSTRACT:
An oligonucleotide library is described that is1 useful for producing an oligonucleotide of preselected sequence comprising a plurality of oligonucleotide members comprising one or more oligonucleotide species and having the compositional formula (X).sub.a (N).sub.b ; wherein X represents a non-degenerate nucleotide base and N represents a degenerate nucleotide base; "a" represents the number of non-degenerate nucleotide positions and is from 3 to 8; "b" represents the number of degenerate nucleotide positions and is from 0 to 4 but not greater that "a"; and wherein each of the oligonucleotide species is capable of forming a hybridization complex with at least one other of the oligonucleotide species in the library such that a single ligation event of the hybridization complex with another hybridization complex derived from the library produces a ligation reaction product comprising greater than 12 contiguous nucleotide base pairs.

REFERENCES:
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patent: 5371006 (1994-12-01), Morgan et al.
patent: 5599921 (1997-02-01), Sorge et al.
patent: 5663062 (1997-09-01), Sorge et al.
Studier. "A Strategy for High-Volume Sequencing of Cosmid DNAs: Random and directed Priming with a Library of Oligonucleotides". Proceedings of the National Academy of Sciences USA 86: 6917-6922 (1989).
Szbalski. "Proposal for Sequencing DNA Using Litigation of Hexamers to Generate Sequential Elongation Primers". Gene 90: 177-178 (1990).

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