Oligonucleotide labeling reactants based on...

Details Oligonucleotide labeling reactants based on... Oligonucleotide labeling reactants based on...

2007-10-16

2007-10-16

Lewis, Patrick (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025300, C536S026100, C536S026800, C536S027100, C536S027130, C536S028100, C536S028600

Reexamination Certificate

active

09985454

ABSTRACT:
The invention relates to a labeling reactant of formula (I) useful for labeling an oligonucleotidewherein: R is a temporary protecting group. A is either a phosphorylating moiety or a solid support tethered to Z via a linker arm E″. Z is a bridge point and is formed fromE is a linker arm between R and Z. E′ is a linker arm between Z and Z′. E″ is a linker arm between Z and A. E′″ is a linker arm between Z′ and G. Z′ is a purine or pyrimidine base. G is a protected bivalent aromatic structure, tethered to two iminodiacetic acid ester groups N(CH2COOR″)2, or G is a structure selected from the group consisting ofor G is a protected functional group, or G is a protected or unprotected organic dye, hapten or a spin label.

REFERENCES:
Hovinen et al. Organic Letters, 2001, vol. 3, pp. 2473-2476.
Augustyns et al., Nucleic Acid Research, 1991, vol. 19, pp. 2587-2593.
Wojczewski et al., “Flourescent Oligonucleotides—Verstile Tools As Probes and Primers for DNA and RNA Analysis,”Synlett1667 (1999).
Beaucage et al., “The Synthesis of Modified Oligonucleotides by the Phosphoramidite Approach and Their Applications,” 49Tetrahedron6123 (1993).

Affiliated with

Also associated with

Rating

Oligonucleotide labeling reactants based on... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oligonucleotide labeling reactants based on..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide labeling reactants based on... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3903255