Oligonucleotide labeling reactants and their use

Details Oligonucleotide labeling reactants and their use Oligonucleotide labeling reactants and their use

2005-09-27

2005-09-27

Wilson, James O. (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025300, C536S025320, C536S026100, C536S026800, C536S027100, C536S027130, C536S028100, C536S028600

Reexamination Certificate

active

06949639

ABSTRACT:
The invention relates to a novel labeling reactant of formula (I) suitable for labeling an oligonucleotidewherein:R is a temporary protecting group. A is either a phosphorylating moiety or a solid support tethered to a bridge point Z via a linker arm E. E′ is a linker arm between G and Z. G is a bivalent aromatic structure, tethered to two iminodiacetic acid ester groups N(COOR′″)2or G is a structure selected from a group consisting ofG is a protected functional group. The invention further concerns a method for direct attachment of a conjugate group to an oligonucleotide structure enabling the attachment of a desired number of these groups during chain assembly. The method comprises a Mitsonobu alkylation.

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