Oligonucleotide for detecting stripe disease resistant rices, an

Details Oligonucleotide for detecting stripe disease resistant rices, an Oligonucleotide for detecting stripe disease resistant rices, an

1996-07-03

1998-07-21

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 536 2432, 536 2433, 935 8, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2104

Patent

active

057833958

ABSTRACT:
There is provided an oligonucleotide for specifically detecting rice plants resistant to stripe disease, said oligonucleotide comprising a sort of nucleic acid sequence 5'-CAGACCGACC-3' SEQ ID NO: 1 and being capable of amplifying a specific DNA fragment of stripe disease resistant rice. There is also provided a method for specifically detecting rice plants resistant to stripe disease, said method comprising the steps of (1) isolating a genomic DNA from rice leaves; (2) amplifying a DNA fragment by polymerase chain reaction; (3) detecting stripe disease resistant rice plants by an agarose electrophoresis.

REFERENCES:
Kurata et. al. Nature Genetics 8:365-372 (Dec. 1994).
Hayakawa et. al. Proc. Natl. Acad. Sci USA 89:9865-9 (Oct. 1992).
Ivell et. al. Proc. Natl. Acad. Sci. USA 81:2006-10 (Apr. 1984).
Stewart, Jr. et. al. Biotechniques 14:748-751 (May 1993).
Linz. Methods in Molecular and Cellular Biology 2:98-102 (1991).
Sealy et. al. in Gel Electrophoresis of Nucleic Acids, edited by Rickwood . al. IRL Press. New York (1990) pp. 51-70.

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